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植物研究  2015 

不同派别杨树无性系SSR遗传距离及聚类分析

DOI: 10.7525/j.issn.1673-5102.2015.01.012, PP. 68-76

Keywords: 杨树,SSR,遗传距离,聚类分析

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Abstract:

?利用SSR分子标记对不同派别的48个杨树无性系进行遗传分析,计算不同无性系间的遗传距离。结果表明:16对引物共检测到81个多态性位点(从100~600bp),不同无性系间的分子遗传距离在0.0617~0.5309,平均距离为0.2504。派内种间遗传距离平均值变化范围为0.1482~0.2741,派间遗传距离平均值变化范围为0.3302~0.4012。聚类分析结果表明在聚类分析自检举值为0.70时,可以清晰的将48个无性系分成白杨派(Leuce)、黑杨派(Aigeiros)、青杨派(Tacamahaca)、大叶杨派(Leucoides)和胡杨派(Turanga)等5类。本研究利用分子手段检测不同派别杨树的遗传距离,为杨树杂交育种、分子育种等提供理论依据。

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