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中国沙漠  2012 

增强UV-B辐射下NO对细胞壁多糖组分含量的影响

Keywords: UV-B辐射,一氧化氮,豌豆幼苗,细胞壁多糖

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Abstract:

以豌豆(Pisumsativum)幼苗茎为材料,研究增强UV-B辐射下一氧化氮(NO)对细胞壁多糖组分含量的影响。结果表明,增强UV-B辐射使单位长度内细胞壁多糖总量增加;使细胞壁多糖结构组分果胶、半纤维素Ⅰ、半纤维素Ⅱ和纤维素的含量增加;NO供体处理对半纤维素Ⅰ、半纤维素Ⅱ和纤维素的影响与UV-B辐射相似,使它们含量增加;增强UV-B辐射条件下,氮合酶(NOS)抑制剂和NO清除剂处理使半纤维素Ⅰ、半纤维素Ⅱ和纤维素的含量降低,抵消了增强UV-B辐射对他们的影响。说明UV-B辐射可能通过NO信号上调了半纤维素Ⅰ、半纤维素Ⅱ和纤维素含量,增加了他们在细胞壁多糖中的比例,因而改变细胞壁的化学特性以及影响了细胞壁的机械延展性。无论对照条件下用NO供体处理,还是UV-B辐射条件下用NOS抑制剂、NO清除剂处理,对果胶含量影响不大,表明NO信号可能与UV-B辐射诱导果胶含量增加无关。

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