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青花菜与甘蓝型油菜小孢子共培养技术研究

DOI: 10.11869/j.issn.100-8551.2015.08.1487, PP. 1487-1493

Keywords: 青花菜,小孢子培养,胚胎发生,共培养

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Abstract:

研究易出胚基因型与难出胚基因型小孢子共培养,可促进难出胚基因型小孢子胚胎发育,提高产胚率。本研究以易出胚甘蓝型油菜(ZS758)基因型与难出胚青花菜(Q1和Q2)基因型为试材进行小孢子混合培养,研究不同数量花蕾混合共培养对青花菜小孢子胚胎诱导效率、胚胎发育过程、胚状体数量及类型和植株再生。结果表明:共培养中青花菜和油菜小孢子胚胎发育过程基本一致;不同处理中,T1-3(2个青花菜Q1花蕾+10个ZS758花蕾)和T2-3(2个青花菜Q2花蕾+10个ZS758花蕾)每蕾平均出胚数分别为12.5个和14.1个、子叶型胚比例分别为78.3%和81.7%、小孢子胚萌发率分别为92.6%和87.6%,均显著高于对照;T1-2(6个青花菜Q1花蕾+6个ZS758花蕾)和T2-2(6个青花菜Q2花蕾+6个ZS758花蕾)获得的青花菜小孢子再生植株数最多,分别为13株和27株;青花菜和油菜小孢子再生植株形态特征差异显著,易于区分。此方法不仅能有效促进难出胚青花菜基因型胚胎发育获得胚状体,而且提高了子叶型胚所占比例,对提高小孢子培养应用效率具有重要意义。

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