全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
核农学报  2014 

美国豆芋地上部位有效成分及α-葡萄糖苷酶抑制活性研究

DOI: 10.11869/j.issn.100-8551.2014.12.2275, PP. 2275-2282

Keywords: 美国豆芋,总黄酮,总皂苷,总糖,抗氧化活性,α-葡萄糖苷酶抑制活性

Full-Text   Cite this paper   Add to My Lib

Abstract:

利用化学显色法扫描分析了美国豆芋不同地上部位(茎、叶、花)中次生代谢物的分布,并比较了3个不同部位提取物主要有效成分含量及体外抗氧化活性和α-葡萄糖苷酶抑制活性。结果表明美国豆芋地上部位(茎、叶、花)中主要含有黄酮、皂苷和多糖等次生代谢产物,且均有不同程度的抗氧化和抑制α-葡萄糖苷酶活性。叶中总黄酮含量最高,花中总皂苷和总糖含量最高。自由基清除能力依次为叶醇提物>花醇提物>茎醇提物;还原力依次为花醇提物>叶醇提物(>茎醇提物。抑制α-葡萄糖苷酶活性则依次为花醇提物>叶醇提物>茎醇提物,不同部位醇提物的活性显著强于其水提物。相关性分析结果显示美国豆芋不同部位的抗氧化活性主要与其黄酮含量相关,而抑制α-葡萄糖苷酶活性则主要与皂苷含量相关(r=0.9941)。研究结果为美国豆芋的综合利用、开发其降血糖花茶提供指导,也对后续美国豆芋花降血糖功能成分的分离纯化和机理研究具有重要意义。

References

[1]  Ni Q, Xu G, Wang Z, Gao Q, Wang S, Zhang Y. Seasonal variations of the antioxidant composition in ground bamboo Sasa argenteastriatus Leaves[J]. International Journal of Molecular Science. 2012, 13: 2249-2262
[2]  Martin A B, Cuadrado Y, Guerra H, Gallego P, Hita O, Martin L, Dorado A, Villalobos N. Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L. [J]. Plant Science, 2000, 154(2): 143-151
[3]  Dawidowicz A, Wianowska D, Olszowy M. On practical problems in estimation of antioxidant activity of compounds by DPPH method (Problems in estimation of antioxidant activity) [J]. Food Chemistry, 2012, 131(3): 1037-1043
[4]  Zheng C, Riccardo B, Guglielimina F. EC50 estimation of antioxidant activity in DPPH assay using several statistical programs [J]. Food Chemistry, 2013, 138(1): 414-420
[5]  Niu Y, Ke D, Yang Q ,Wang X, Chen Z, An X, Shen W. Temperature-dependent stability and DPPH scavenging activity of liposomal curcumin at pH 7.0[J]. Food Chemistry, 2012, 135(3): 1377-1382
[6]  Catarina G, Helena M A, Maria S G, Xavier M. Optimization of ABTS radical cation assay specifically for determination of antioxidant capacity of intracellular extracts of microalgae and cyanobacteria[J]. Food Chemistry, 2013, 138(1): 638-643
[7]  Lou H, Hu Y, Zhang L, Sun P, Lu H. Nondestructive evaluation of the changes of total flavonoid, total phenols, ABTS and DPPH radical scavenging activities, and sugars during mulberry (Morus alba L.) fruits development by chlorophyll fluorescence and RGB intensity values[J]. LWT - Food Science and Technology, 2012, 47(1): 19-24
[8]  Gkhan D. Freeze-dried ABTS+ method: A ready-to-use radical powder to assess antioxidant capacity of vegetable oils[J]. Food Chemistry, 2012, 133(4): 1658-1663
[9]  孙勃, 许映君, 袁高峰, 郭容芳, 魏佳, 李振, 刘娜, 汪俏梅. 花椰菜主要生物活性物质及其抗氧化能力分析[J]. 核农学报, 2010, 24(2): 330-335
[10]  杨少辉, 宋英今, 王洁华, 季静. 雪莲果体外抗氧化和自由基清除能力[J]. 食品科学, 2010, 31(17): 166-169
[11]  Dong H, Li M, Zhu F, Liu F, Huang J. Inhibitory potential of trilobatin from Lithocarpus polystachyus Rehd against a-glucosidase and a-amylase linked to type 2 diabetes[J]. Food Chemistry, 2012, 130(2):261-266
[12]  Frankel E N, Meyer A S. The problems of using one dimensional methods to evaluate multifunctional food and biological antioxidants[J]. Journal of Science and Food Agriculture, 2000, 80(13): 1925-1941
[13]  Krentz A J, Bailey C J. Oral antidiabetic agents: Current role in type 2 diabetes mellitus[J]. Drugs, 2005, 65(3): 385-411
[14]  Zhang Y, Kouzuma Y, Miyaji T, Yonekura M. Purification, characterization, and cDNA cloning of a bowman-birk type trypsin inhibitor from Apios Ameicana Medikus Tubers [J]. Bioscience Biotechnology and Biochemistry, 2008, 72(1): 171-178
[15]  Zhang Y, Zhou C, Tang S, Yu X, Kouzuma Y, Yonekura M. Effect of AATI, a Bowman-Birk type inhibitor from Apios americana, on proliferation of cancer cell lines [J]. Food Chemistry, 2011, 128(4): 909-915
[16]  Nara K, Nihei K, Ogasawara Y, Koga H, Kato Y. Novel isoflavone diglycoside in groundnut (Apios americana Medik) [J]. Food Chemistry, 2011, 124(3): 703-710
[17]  Takashima M, Nara K, Niki E, Yoshida Y, Hagihara Y, Stowe M, Horie M. Evaluation of biological activities of a groundnut (Apios americana Medik) extract containing a novel isoflavone [J]. Food Chemistry, 2013, 138(1):298-305
[18]  Juliarni, Hoshikawa K. Characteristics of dry matter production and yield of Apios (Apios americana Medikus) clones[J]. Japanese Journal of Crop Science, 1996, 65(1): 98-102
[19]  Juliarni, Hoshikawa K. The growth of Apios (Apios americana Medikus), a new crop, under field conditions [J]. Japanese Journal of Crop Science, 1995, 64(2): 323-327
[20]  Iwai K, Matsue H. Ingestion of Apios americana Medikus tuber suppresses blood pressure and improves plasma lipids in spontaneously hypertensive rats [J]. Nutrition Research, 2007, 27(4): 218-224
[21]  陈玉婷, 杨云, 王英华. 常用中药化学鉴定[M]. 北京: 化学工业出版社, 2005
[22]  郝海燕, 沙爱龙. 硬枝碱蓬化学成分的定性分析及生物碱含量的测定[J]. 食品科技, 2013, 38(1): 244-247
[23]  王萍, 朱祝军. 腌制加工对不同品种叶用芥菜抗氧化物质含量和抗氧化活性的影响[J]. 核农学报, 2006, 20(6): 516-520
[24]  Ni Q, Zhang Y,Xu G, Gao Q, Gong L, Zhang Y. Influence of harvest season and drying method on the antioxidant activity and active compounds of two bamboo grass leaves[J]. Journal of food processing and preservation. 2014, 38(4):1565-1576
[25]  Chen Y, Xie M, Gong X. Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum [J]. Journal of Food Engineering, 2007, 81(1): 162-170

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133