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坐骨神经缺损后大鼠近端神经新miRNA的鉴定与功能分析

, PP. 525-531

Keywords: miRNA,背根节,大鼠,Solexa

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Abstract:

周围神经系统在损伤后能够再生,再生过程中许多基因和蛋白的表达发生改变.miRNA是一类进化上高度保守、能够在转录后调控基因表达的非编码RNA.本研究聚焦在大鼠坐骨神经缺损后,近端神经新miRNA的鉴定和功能注解.通过深度测序、计算机分析和Q-PCR验证,98个新miRNA在坐骨神经缺损后的0,1,4,7和14天被发现和鉴定.进一步预测了这些miRNA的靶基因,分析了靶基因参与的生物学过程,结果显示靶基因参与的生物学过程与坐骨神经缺损后神经损伤和再生的过程基本一致.本实验为miRNA在坐骨神经缺损后的调控作用的研究提供了基础,有助于周围神经损伤和再生分子机制的进一步研究.

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