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高效液相色谱法同时测定人血浆中洛匹那韦和利托那韦浓度

, PP. 326-332

Keywords: 洛匹那韦,利托那韦,高效液相色谱法,紫外检测,血药浓度

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Abstract:

为建立同时测定人血浆中洛匹那韦和利托那韦浓度的高效液相色谱方法,取血浆样品200μL,以地西泮为内标,叔丁基甲醚液提取,有机相室温下氮气吹干,20%乙腈100μL复溶,振荡离心后取20μL进样分析.结果发现,洛匹那韦在0.5~20mg/L范围内、利托那韦在0.05~5mg/L范围内线性良好(r=0.9995和0.9997).低(1/0.25mg/L)、中(10/2.5mg/L)和高(18/4.5mg/L)3个质量浓度质控样品中,洛匹那韦和利托那韦的日内变异分别为2.16%~3.20%和2.12%~2.60%,日间变异分别为2.34%~4.04%和0.31%~4.94%.方法学回收率为99.88%~106.29%.利托那韦、洛匹那韦和内标(地西泮)的绝对提取率分别为79.17%,52.26%和91.35%.稳定性考察结果良好.结果显示,本方法灵敏度高、经济简便、结果准确可靠,为开展洛匹那韦和利托那韦的人体药代动力学研究提供了方法学基础.

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