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稻瘟病新隐性抗病基因pi55(t)的遗传及定位

, PP. 125-134

Keywords: 稻瘟病,隐性抗病基因,pi55(t),抗性遗传,基因定位

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Abstract:

粤晶丝苗2号是广东省优质稻主栽品种和省区试对照品种,具有高度的稻瘟病抗性.用中国不同地区297个稻瘟病菌株进行抗谱分析,粤晶丝苗2号对广东、四川、辽宁和黑龙江稻瘟病菌群体的抗性频率均达到100%,其抗谱较主要抗源品种三黄占和28占更广.利用不同的菌株,对粤晶丝苗2号/露明的F2群体和F4株系进行抗性分离分析,结果表明,该品种的稻瘟病抗性由显、隐性的多基因控制.通过基于F4单基因分离株系的BSA分析,分别在染色体2,6,8和12上筛选到了与其抗病基因连锁的候选标记.通过基于基因组位置已知的分子标记的连锁分析,将其中的隐性基因定位于染色体8约1.9cM/152kb的区域内.由于该区域内尚未有主效抗病基因的存在,因此,该基因是新的隐性抗病基因,被命名为pi55(t).通过对该区域内候选基因的注释,发现其中编码LRR蛋白的Os08g40090和编码重金属结合域蛋白的Os08g40130可能是其最佳的候选基因.

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