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对核启动子具有增强功能的TinⅡ内含子在茎瘤芥中影响胞质雄性不育基因T的功能

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Keywords: 胞质雄性不育性,选择性剪接,增强子,内含子

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Abstract:

从茎瘤芥胞质雄性不育系(CMS)线粒体中克隆的T基因是一个CMS相关基因,它能够通过选择性剪接产生T1170和T1243两个转录本,而T1243是T基因在转录过程中保留了内含子TinⅡ的产物.本实验室前期的实验结果表明,在模式植物拟南芥中过表达T1243(OE-T1243)后,植株表现出明显的雄性不育表型,而OE-T1170植株的表型与野生型无异,然而这一现象的机制却不明确.本研究通过花药石蜡切片进一步确认了OE-T1243植株异于OE-T1170植株的花粉败育表型.对15个已知花药发育相关基因的表达分析表明,与野生型相比,OE-T1243植株中nozzle/sporocyteless,barelyanymeristem1和2发生了不同程度的表达下调,而在OE-T1170中未发生明显变化.为了进一步研究TinⅡ内含子的潜在功能,将其构建于(-60)35S基本启动子区域上游,来测试对下游GUS基因的启动效果.对转化植株的分析表明,TinⅡ具有增强子功能,它可以显著增强GUS基因的表达.表明TinⅡ内含子能够作为一个增强子影响CMS相关基因T的功能.

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