A novel glassy carbon electrode (GCE) modi？ed with carbon-spheres has been fabricated through a simple casting procedure. The modified GCE displays high selectivity and excellent electrochemical catalytic activities towards dopamine (DA), serotonin (5-HT), and ascorbic acid (AA). In the co-existence system, the peak separations between AA and DA, DA and 5-HT, and AA and 5-HT are large up to 230, 180, and 410 mV, respectively. Differential pulse voltammetry (DPV) has been employed to simultaneously detect DA, 5-HT, and AA, and the linear calibration curves for DA, 5-HT, and AA are obtained in the range of 20.0–150.0 μM, 40.0–750.0 μM and 300.0–2,000.0 μM with detection limits (S/N = 3) of 2.0 μM, 0.7 μM and 0.6 μM, respectively. The proposed electrode has been applied to detect DA, 5-HT, and AA in real samples using standard addition method with satisfactory results.
References
[1]
Michael, D.J.; Wightman, R.M. Electrochemical monitoring of biogenic amine neurotransmission in real time. J. Pharm. Biomed. Anal. 1999, 19, 33–46.
[2]
Cooper, J.R.; Bloom, F.E.; Roth, R.H. The Biochemical Basis of Neuropharmacology; Oxford University Press: Oxford, UK, 1982.
[3]
Wightman, R.M.; May, L.J.; Michael, A.C. Detection of dopamine dynamics in the brain. Anal. Chem. 1998, 70, 769A–779A.
[4]
Mo, J.W.; Ogorevc, B. Simultaneous measurement of dopamine and ascorbate at their physiological levels using voltammetric microprobe based on overoxidized poly (1,2-phenylenediamine)-coated carbon fiber. Anal. Chem. 2001, 73, 1196–1202.
[5]
Gershon, M.D. The Second Brain; Harper Collins: New York, NY, USA, 1998.
[6]
Kema, I.P.; Meijer, W.G.; Meiborg, G.; Ooms, B.; Willemse, P.H.B.; de Vries, E.G.E. Profiling of tryptophan-related plasma indoles in patients with carcinoid tumors by automated, on-line, solid-phase extraction and hplc with fluorescence detection. Clin. Chem. 2001, 47, 1811–1820.
[7]
Rice, M.E. Ascorbate regulation and its neuroprotective role in the brain. Trends Neurosci. 2000, 23, 209–216.
Hoyer, B.; Jensen, N. Stabilization of the voltammetric serotonin signal by surfactants. Electrochem. Commun. 2006, 8, 323–328.
[10]
Atta, N.F.; El-Kady, M.F. Novel poly(3-methylthiophene)/Pd, Pt nanoparticle sensor: Synthesis, characterization and its application to the simultaneous analysis of dopamine and ascorbic acid in biological fluids. Sens. Actuators B: Chem. 2010, 145, 299–310.
[11]
Perry, M.; Li, Q.; Kennedy, R.T. Review of recent advances in analytical techniques for the determination of neurotransmitters. Anal. Chim. Acta. 2009, 653, 1–22.
[12]
Stamford, J.A.; Justice, J.B. Probing brain chemistry: Voltammetry comes of age. Anal. Chem. 1996, 69, 359A–363A.
[13]
Selvaraju, T.; Ramaraj, R. Electrochemically deposited nanostructured platinum on Nafion coated electrode for sensor applications. J. Electroanal. Chem. 2005, 585, 290–300.
[14]
Selvaraju, T.; Ramaraj, R. Simultaneous determination of ascorbic acid, dopamine and serotonin at poly(phenosafranine) modified electrode. Electrochem. Comm. 2003, 5, 667–671.
[15]
Selvaraju, T.; Ramaraj, R. Simultaneous determination of dopamine and serotonin in the presence of ascorbic acid and uric acid at poly(o-phenylenediamine) modified electrode. J. Appl. Electrochem. 2003, 33, 759–762.
[16]
Jin, G.P.; Lin, X.Q.; Gong, J.M. Novel choline and acetylcholine modified glassy carbon electrodes for simultaneous determination of dopamine, serotonin and ascorbic acid. J. Electroanal. Chem. 2004, 569, 135–142.
[17]
Jiang, X.; Lin, X. Overoxidized polypyrrole film directed DNA immobilization for construction of electrochemical micro-biosensors and simultaneous determination of serotonin and dopamine. Anal. Chim. Acta 2005, 537, 145–151.
[18]
Lu, L.; Wang, S.; Lin, X. Attachment of DNA to the carbon fiber microelectrode via gold nanoparticles for simultaneous determination of dopamine and serotonin. Anal. Sci. 2004, 20, 1131–1136.
[19]
Wei, J.; He, J.B.; Cao, S.Q.; Zhu, Y.W.; Wang, Y.; Hang, G.P. Enhanced sensing of ascorbic acid, dopamine and serotonin at solid carbon paste electrode with a nonionic polymer film. Talanta 2010, 83, 190–196.
[20]
Oni, J.; Nyokong, T. Simultaneous voltammetric determination of dopamine and serotonin on carbon paste electrodes modified with iron(II) phthalocyanine complexes. Anal. Chim. Acta 2001, 434, 9–21.
[21]
Anastassiou, C.A.; Patel, B.A.; Arundell, M.; Yeoman, M.S.; Parker, K.H.; O'Hare, D. Subsecond voltammetric separation between dopamine and serotonin in the presence of ascorbate. Anal. Chem. 2006, 78, 6990–6998.
[22]
Wu, K.; Fei, J.; Hu, S. Simultaneous determination of dopamine and serotonin on a glassy carbon electrode coated with a film of carbon nanotubes. Anal. Biochem. 2003, 318, 100–106.
[23]
Wang, Z.H.; Liang, Q.L.; Wang, Y.M.; Luo, G.A. Carbon nanotube-intercalated graphite electrodes for simultaneous determination of dopamine and serotonin in the presence of ascorbic acid. J. Electroanal. Chem. 2003, 540, 129–134.
[24]
Miyazaki, K.; Matsumoto, G.; Yamada, M.; Yasui, S.; Kaneko, H. Simultaneous voltammetric measurement of nitrite ion, dopamine, serotonin with ascorbic acid on the GRC electrode. Electrochim. Acta 1999, 44, 3809–3820.
[25]
Kachoosangi, R.B.; Compton, R.G. A simple electroanalytical methodology for the simultaneous determination of dopamine, serotonin and ascorbic acid using an unmodified edge plane pyrolytic graphite electrode. Anal. Bioanal. Chem. 2007, 387, 2793–2800.
[26]
Rand, E.; Periyakaruppan, A.; Tanaka, Z.; Zhang, D.A.; Marsh, M.P.; Andrews, R.J.; Lee, K.H.; Chen, B.; Meyyappan, M.; Koehne, J.E. A carbon nanofiber based biosensor for simultaneous detection of dopamine and serotonin in the presence of ascorbic acid. Biosens. Bioelectron. 2013, 42, 434–438.
[27]
Gao, F.; Guo, X.; Yin, J.; Zhao, D.; Li, M.; Wang, L. Electrocatalytic activity of carbon spheres towards NADH oxidation at low overpotential and its applications in biosensors and biofuel cells. RSC Adv. 2011, 1, 1301–1309.
[28]
Zhao, D.; Guo, X.; Gao, Y.; Gao, F. An electrochemical capacitor electrode based on porous carbon spheres hybrided with polyaniline and nanoscale ruthenium oxide. ACS Appl. Mater. Interfaces 2012, 4, 5583–5589.
[29]
Wu, G.; Guo, X.; Zhou, J.; Zhao, D.; Gao, Y.; Li, M.; Gao, F. Fabrication of porous carbon spheres/manganese oxide composite electrodes for electrochemical capacitors. ECS Solid State Lett. 2012, 1, M8–M12.
[30]
Wu, G.; Zhou, J.; Jiang, X.; Guo, X.; Gao, F. Electrochemical detection of low-molecular-mass biothiols in biological fluids at carbon-spheres-modified glassy carbon electrodes. Electrocatalysis 2013, 4, 17–23.
