全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

Calcium Wave Propagation in Networks of Endothelial Cells: Model-based Theoretical and Experimental Study

DOI: 10.1371/journal.pcbi.1002847

Full-Text   Cite this paper   Add to My Lib

Abstract:

In this paper, we present a combined theoretical and experimental study of the propagation of calcium signals in multicellular structures composed of human endothelial cells. We consider multicellular structures composed of a single chain of cells as well as a chain of cells with a side branch, namely a “T” structure. In the experiments, we investigate the result of applying mechano-stimulation to induce signaling in the form of calcium waves along the chain and the effect of single and dual stimulation of the multicellular structure. The experimental results provide evidence of an effect of architecture on the propagation of calcium waves. Simulations based on a model of calcium-induced calcium release and cell-to-cell diffusion through gap junctions shows that the propagation of calcium waves is dependent upon the competition between intracellular calcium regulation and architecture-dependent intercellular diffusion.

References

[1]  Bagher P, Segal SS (2011) Regulation of Blood Flow In the Microcirculation: Role of Conducted Vasodilation. Acta Physiol 202: 271–284. doi: 10.1111/j.1748-1716.2010.02244.x
[2]  Bissell MJ, Rizki A, Mian IS (2003) Tissue architecture: the ultimate regulator of breast epithelial function. Curr Opin Cell Biol 15: 753–762. doi: 10.1016/j.ceb.2003.10.016
[3]  Hansen RK, Bissell MJ (2000) Tissue architecture and breast cancer: The role of extracellular matrix and steroid hormones. Endocrine-Related Cancer 7: 95–113. doi: 10.1677/erc.0.0070095
[4]  Kirshner J, Chen CJ, Liu P, Huang J, Shively JE (2003) CEACAM1-4S, a cell-cell adhesion molecule, mediates apoptosis and reverts mammary carcinoma cells to a normal morphogenic phenotype in a 3D culture. Proc Natl Acad Sci USA 100: 521–526. doi: 10.1073/pnas.232711199
[5]  Davidson LA (2008) Integrating morphogenesis with underlying mechanics and cell biology. Curr Top Dev Biol 81: 113–133. doi: 10.1016/s0070-2153(07)81003-9
[6]  Tran QK, Ohashi K, Watanabe H (2000) Calcium signaling in endothelial cells. Cardiovascular Research 48: 13–22. doi: 10.1016/s0008-6363(00)00172-3
[7]  Watts DJ, Strogatz S (1998) Collective dynamic of “small-world” networks. Nature 393: 440–442. doi: 10.1038/30918
[8]  Eray M, Deymier PA, Hoying JH, Runge K, Vasseur JO (2008) Resonant filtering of compositional waves in multicellular networks. Physica D 237: 2777. doi: 10.1016/j.physd.2008.04.020
[9]  Deymier PA, Eray M, Hoying JH, Runge K, Vasseur JO (2010) Architecture-dependent signal conduction in model networks of endothelial cells. Phys Rev E Stat Nonlin Soft Matter Phys 81: 041915. doi: 10.1103/physreve.81.041915
[10]  Deymier PA, Hoying JH, Runge K, Vasseur JO (2010) Calcium wave propagation in chains of endothelial cells with non-linear reaction dynamics: green's function approach. Phys Rev E 82: 041913. doi: 10.1103/physreve.82.041913
[11]  Ali MH, Schumacker PT (2002) Endothelial Responses to Mechanical Stress: Where Is The Mechanosensor? Crit Care Med 30 (5) S198–S206. doi: 10.1097/00003246-200205001-00005
[12]  Drumheller PD, Hubbell JA (1991) Local modulation of intracellular calcium levels near a single-cell wound in human endothelial monolayers. Arteriol Thromb 11: 1258–1265. doi: 10.1161/01.atv.11.5.1258
[13]  Junkin M, Leung SL, Whitman S, Gregorio CC, Wong PK (2011) Cellular self-organization via autocatalytic alignment feedback. J Cell Sci 124: 4213–4220. doi: 10.1242/jcs.088898
[14]  Junkin M, Wong PK (2011) Probing cell migration in confined environments by plasma lithography. Biomaterials 32: 1848–1855. doi: 10.1016/j.biomaterials.2010.11.009
[15]  Keyes J, Junkin J, Cappello J, Wu X, Wong PK (2008) Evaporation-induced assembly of biomimetic polypeptides. Appl Phys Lett 93: (023120)1–(023120)3. doi: 10.1063/1.2957992
[16]  Junkin M, Watson J, Vande Geest JP, Wong PK (2009) Template-guided self-assembly of colloidal quantum dots using plasma lithography. Adv Mater 21: 1247–1251. doi: 10.1002/adma.200802122
[17]  Junkin M, Leung SL, Yang Y, Lu Y, Volmering J, et al. (2011) Plasma Lithography Surface Patterning For Creation of Cell Networks. J Vis Exp 14;(52). pii: 3115 doi: 10.3791/3115.
[18]  Bezprozvanny I, Watras J, Ehrlich BE (1991) Bell-shaped calcium-response curves of IP3 and calcium-gated channels from endoplasmic reticulum of cerebellum. Nature 351: 751–754. doi: 10.1038/351751a0
[19]  Meissner,G (1994) Ryanodine receptor/Ca2+ release channels and their regulation by endogenous effectors. Annu Rev Physiol 56: 485–508. doi: 10.1146/annurev.ph.56.030194.002413
[20]  Combettes GL, Leybaert L (2007) Calcium dynamics: spatio-temporal organization from the subcellular to the organ level. Int Rev Cytol 261: 193–245. doi: 10.1016/s0074-7696(07)61005-5
[21]  Finch EA, Turner TJ, Goldin SM (1991) Calcium as a coagonist of inositol 1,4,5-trisphosphate-induced calcium release. Science 252: 443–446. doi: 10.1126/science.2017683
[22]  Hofer T, Venance L, Giaume C (2002) Control and plasticity of intercellular calcium waves in astrocytes: a modeling approach. J Neurosci 22 (12) 4850–4859.
[23]  Bagher P, Davis, M J, Segal, S S (2011) Visualizing Calcium Responses to Acetylcholine Convection Along Endothelium of Arteriolar Networks in Cx40BAC-GCaMP2 Transgenic Mice. Am J Physiol Heart Circ Physiol 301 (3) H794–802. doi: 10.1152/ajpheart.00425.2011
[24]  Allbritton NL, Meyer T, Stryer L (1992) Range of Messenger Action of Calcium Ion and Inositol 1,4,5-Trisphosphate. Science 258: 1812–1815. doi: 10.1126/science.1465619
[25]  Goldbeter A, Dupont G, Berridge MJ (1990) Minimal Model For Signal-Induced Calcium Oscillations and For Their Frequency Encoding Through Protein Phosphorylation. Proc Natl Acad Sci USA 87: 1461–1465. doi: 10.1073/pnas.87.4.1461
[26]  Dupont G, Berridge MJ, Goldbeter A (1991) Signal-Induced Ca2+ Oscillations: Properties of A Model Based On Ca2″-Induced Ca2+ Release. Cell Calcium 12: 73–86. doi: 10.1016/0143-4160(91)90010-c

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133