Additives Promote Adventitious Buds Induction from Stem Segments of Bitter Melon (Momordica charantia L.)

The effects of thidiazuron (TDZ), silver nitrate (AgNO3) and triacontanol on adventitious buds induction from stems of balsam pear (Momordica charantia L.) were investigated. It was found that TDZ was necessary for bud development and the higher concentration of it could induce adventitious buds efficiently, while 0.1 mg/L was the best concentration to induce adventitious buds considered effect and cost. Bud formation was significantly affected by AgNO3 and triacontanol. Best results were obtained at concentration of 2.0 mg/L separately.