DETERMINATION OF NATURAL COMPOUNDS IN DASHMOOL EXTRACTS BY THIN LAYER CHROMATOGRAPHY AND HIGH-PRESSURE LIQUID CHROMATOGRAPHY

The determination of the natural compounds lupeol, beta sitosterol and stigmasterol in plant extracts using HPLC Shimadzu Model LC2010 AHT Auto Sampler (UV –VIS Detector) is reported. The methods were applied to the analysis of lupeol, beta sitosterol and stigmasterol in petroleum ether extract originating from Dashmool. The result of TLC of Dashmool petroleum ether extract showed the presence of β- sitosterol, stigmasterol and lupeol in Dashmool plant extract at different Rf. HPLC profile of petroleum ether extract of Dashmool have characteristics peaks at retention time 2.888, 2.971, 3.135, 3.442, 4.018, 4.220, 4.406, 4.885, 5.388, 5.657, 6.083 (Stigmasterol), 7.848, 9.137, 10.012, 17.656 (Lupeol), 18.138 (β-Sitosterol) and 23.096. These peaks showed that there are different compounds and characteristic fingerprints of drug to judge in a herbal formulation.