Docosahexaenoic acid has influence on action potentials and transient outward potassium currents of ventricular myocytes

The calcium-tolerant rat ventricular myocytes were isolated by enzyme digestion. Action potentials and Ito of epicardial, mid-cardial and endocardial ventricular myocytes were recorded by whole-cell patch clamp technique.1. Action potential durations (APDs) were prolonged from epicardial to endocardial ventricular myocytes (P < 0.05). 2. Ito current densities were decreased from epicardial to endocardial ventricular myocytes, which were 59.50 ± 15.99 pA/pF, 29.15 ± 5.53 pA/pF, and 12.29 ± 3.62 pA/pF, respectively at +70 mV test potential (P < 0.05). 3. APDs were gradually prolonged with the increase of DHA concentrations from 1 μmol/L to 100 μmol/L, however, APDs changes were not significant as DHA concentrations were in the range of 0 μmol/L to 1 μmol/L. 4. Ito currents were gradually reduced with the increase of DHA concentrations from 1 μmol/L to 100 μmol/L, and its half-inhibited concentration was 5.3 μmol/L. The results showed that there were regional differences in the distribution of action potentials and Ito in rat epicardial, mid-cardial and endocardial ventricular myocytes. APDs were prolonged and Ito current densities were gradually reduced with the increase of DHA concentrations.The anti-arrhythmia mechanisms of DHA are complex, however, the effects of DHA on action potentials and Ito may be one of the important causes.Fatty acids, especially polyunsaturated fatty acids (PUFAs), play an important role in life and death of cardiac cells. Reasons are as follow: 1. they are essential fuels for mechanical, electrical, and synthetic activities of the heart; 2. their levels are abnormally high in an ischemia followed by a reperfusion; and 3. dietary fish oil is apparently beneficial for heart function [1,2]. Therefore, the beneficial effects of PUFAs on cardiovascular diseases, such as fish oil, have been reported, and the effects of PUFAs on anti-arrhythmias and prevention of sudden death have been highlights [3-6]. It has been reported that PUFAs have the ro