Isolation and identification of yeasts in milk samples from cows' mammary glands

The purpose of this study was to isolate fungi from the milk of cow udder quarters with clinical mastitis. The samples were delivered in Veterinary laboratory in Kri evci during a routine mastitis diagnostics. Milk samples were cultured on Columbia agar (Merck, KgaA, Darmstadt, Germany) with 5 % ovine blood, Sabouraud 4 % maltose agar (Merck, KgaA, Darmstadt, Germany) and Rice extract agar (Merck, KgaA, Darmstadt, Germany). The final diagnosis was established regarding to the results of the API 20 C AUX systems (bioMerieux, Lyon, France). All of the fungal isolates were yeasts, genera Candida spp. (76.2 %) and Trichosporon spp. (23.8 %). The most prevalent species were: C. quilliermondi (21.4 %), C. krusei/inconspicua (11.9 %) and Trichosporon mucoides (14.3 %).