Isolation and Purification of the Active Principle from Agkistrodon Acutus Snake Venom by High Performance Ion Exchange Liquid Chromatography
高效离子交换色谱法分离皖南尖吻蝮蛇毒活性组分

The active component from Agkistrodon acutus snake venom was further purified by high performance ion-exchange liquid chromatography over a Protein-PAK DEAE-8HR column(10.0 mm i.d. x 100 mm). The elution was operated with a linear gradient from 10% to 100% NaCl-0.02 mol/L Tris-HCl buffer(pH 8.0). The quantitative results were calculated as 45.41% based on peak area nomalization and the purity of the active component was 90% determined by electrophoresis.