Determination of trace 4,4''-diaminobiphenyl, 4-nitrophenol and phenol in environmental water by high performance liquid chromatography]
高效液相色谱法测定环境水中痕量4,4′-二氨基联苯、4-硝基酚和苯酚

A method for simultaneous determination of trace 4,4'-diaminobiphenyl, 4-nitrophenol and phenol in waste water by high performance liquid chromatography with spectrophotometric detection is reported. These components were separated on a Phenomenex Spherex C18 column with V(acetonitrile): V(ether): V(50 mmol.L-1 acetate) = 12:10:78 buffer (pH 6.0) as mobile phase and UV detection at 275 nm. The detection limits were 0.14, 0.19 and 0.08 ng for 4,4'-diaminobiphenyl, 4-nitrophenol and phenol, respectively, when the ratio of signal to noise was 2. The relative standard deviation was < 2.2 (n = 6). This method is sensitive and has been applied to the analysis of environmental water samples with satisfactory results.