Determination of Alkaloids in Pericarpium Papaveris(Yingsuqiao) by High Performance Capillary Electrophoresis
高效毛细管电泳法测定罂粟壳中生物碱的含量

Codeine, morphine and papaverine in Pericarpium papaveris (Yingsuqiao) were determined by HPCE. These three compounds in Pericarpium papaveris were entirely separated. The HPCE conditions were as follows: buffer: 50 mmol/L phosphate, pH 7.0; V = 25 kV; l(eff.) = 50 cm; L = 64.5 cm; i.d. = 50 microm; lambda = 212 nm; T = 25 degrees C; sample size = 15 kPa x s. The recoveries of codeine, morphine and papaverine were 96.61%, 95.90% and 95.37% respectively, which shows that this method is simple, accurate and reproducible and can be used for the quality control of the Chinese herbal medicine.