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色谱 2010
Fast determination of active components in Angelica dahurica extract using capillary electrochromatography with methacrylate ester-based monolithic columns
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Abstract:
The separation and determination of four important active components(imperatorin,isoimperatorin,phelloptorin and falcarindiol) from Angelica dahurica extract has been performed using capillary electrochromatography(CEC) with a methacrylate ester-based monolithic column.The effect of the porogen ratio on the column preparation was studied.The mobile phase composition,such as the concentration of organic solvent,the ionic strength and the pH of the buffer were also optimized.Under the optimized conditions(50%...