Application of online two-dimensional separation system using monolithic columns for proteome analysis of human cartilage
毛细管整体柱在线二维分离系统应用于人体软骨的蛋白质组分析

In Shotgun proteome analysis,where nano-flow is adopted to increase the sensitivity as well as extremely complicated samples such as proteolytic digest are inevitably confronted,monolithic capillary columns are widely used to improve the liquid chromatography separation performance.It is known that cartilage contains extensive amounts of extracellular matrix(ECM),in which collagens and aggrecans being the most abundant macromolecules.It is obvious that the high content of ECM components causes a challenge i...