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色谱 2010
Optimization of two-dimensional high performance liquid chromatographic columns for highly efficient separation of intact proteins
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Abstract:
In order to optimize two-dimensional liquid chromatographic(2D-LC) columns for highly efficient separation of proteins,several liquid chromatographic columns were investigated and evaluated.Weak anion-exchange(WAX) column was chosen as the first dimension because of its extensive protein separation power.By comparison of different WAX chromatographic columns for human liver protein separation,TSKgel DEAE-5PW column was selected as the first dimension of a 2D-LC system.For the second dimension,ten typical re...
