Optimization of L-glutamic acid racemization reaction with capillary array electrophoresis
毛细管阵列电泳技术用于L-谷氨酸消旋化反应的条件优化

L-Glutamic acid (Glu) racemization research is of great importance for the preparation of optical pure D-Glu.In the present work,we studied L-Glu racemization reaction through Schiff base intermediate by using capillary array electrophoresis (CAE) with 532 nm laser-induced confocal fluorescence detection.The racemization products were labeled by carboxytetramethylrhodamine succinimidyl ester (TAMRA) and analyzed in a 100 mmol/L Tris-borate buffer (pH 10) system containing 2 mmol/L β-CD by CAE.Under this condition,the TAMRA-Glu enantiomer can be completely separated.The influences of the type and dosage of the aldehyde,organic acids,as well as the water content in the reaction system on the racemization were examined in detail,and L-Glu was racemized quickly in the presence of 0.2 molar ratio of L-Glu to salicylaldehyde (as the catalyzer) in acetic acid containing 20%(v/v) water.