A New Method for Separation of 2,4,6-Trinitrotoluene(TNT) and Its Metabolites by High Performance Liquid Chromatography(HPLC)
一种新的分离三硝基甲苯及其代谢产物的高效液相色谱方法

n HPLC method is described for separation of TNT and its main reduction metabolites. Two columns(LC-C(18) and LC-CN)are connected in series and operated isocratically at 1.0mL/min with water-methanol-tetrahydrofuran(60 35 5 ). The baseline separation of 4-amino-2,6-dinitrotoluene(4A) and 2-amino-4,6-dinitrotoluene(2A) ,which are main metabolites of TNT,is obtained(Rs=2.1). All the TNT and itsmetabolites,including TNT,2,6-diamino-4-nitrotoluene(2,6-DA),2,4-diamino-6-nitrotoluene(2,4-DA),4-hydroxylamino-2, 6-Ainitrotoluene(4HA), 4A and 2A , can be separated from each other with this method.