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色谱 2001
Determination of Salicylic Acid and Its Hydroxylated Products Using High Performance Liquid Chromatography and Fluorescence Detection
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Abstract:
High performance liquid chromatography (HPLC) with fluorescence detector was used to determine salicylic acid (SA) and its main hydroxylated products, 2,3 dihydroxybenzoic acid (2,3 DHBA) and 2,5 dihydroxybenzoic acid (2,5 DHBA). Mobile phase was 30 mmol/L acetate(pH 4.9) and the flow rate was 1.0 mL/min. Low detection limits, 10 nmol/L for 2,3 DHBA, 0.6 nmol/L for 2,5 DHBA and 1.0 nmol/L for SA were obtained. The photolysis system of salicylic acid and H\-2O\-2 was investigated. The products in the system were measured and the yields were 45% and 51% for 2,3 DHBA and 2,5 DHBA, respectively.
