Determination of Two Components in Chloramphenicol and Hydrocortisone Ear Drops by High Performance Liquid Chromatography
HPLC法测定氯霉素氢化可的松滴耳液中两组分的含量

Chloramphenicol and hydrocortisone ear drops are possessed of anti-inflammatory and antiallergic functions. It can be used effectively in the treatment of tympanitis. It is composed of chloramphenicol and hydrocortisone. In this paper, we report a reversed phase HPLC method for the determination of chloramphenicol and hydrocortisone in the drops. The operatings conditions were YWG-C18 column (5 microns, 5 mm x 150 mm) at 30 degrees C, mobile phase of methanol-water (60:40, V/V) and UV detector at 244 nm. Prednisone acetate was selected as the internal standard. The linear relationship of calibration curves was good in the range of 40-200 and 8-40 mg/L for chloramphenicol and hydrocortisone, respectively (r = 0.9999). The average recovery and RSD of chloramphenicol and hydrocortisone were 99.91%, 0.86% and 99.58%, 1.34% (n = 6) respectively. The total time for separation and determination was within 9 min. The method was simple, sensitive, rapid and accurate.