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色谱  2001 

The Analysis of Rice Total RNA by Capillary Electrophoresis
毛细管电泳技术分析水稻中的总核糖核酸

Keywords: capillary electrophoresis,rice total RNA,rice tRNA
毛细管电泳
,水稻,总RNA,转移RNA,分析,生物化学

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Abstract:

When the RNase was eliminated through backing at 200 degrees C or treated by diethylpyrocarbonate (DEPC), the total RNA of rice could be separated within 15 min using 1.0%T, 0%C linear polyacrylamide as sieving matrix and 7 mol/L urea as denaturant. The tRNA of rice can be separated into two classes and about nine peaks when high concentrated polyacrylamide sieving matrix (5.0%T, 0%C) was used. This technique could provide one of the rapid and accurate methods for the determination of RNA in plants.

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