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色谱 2001
Determination of Syringin and Rutin in 11 Species of Saussurea by High Performance Liquid Chromatography
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Abstract:
Zorbax ODS column and gradient elution of acetonitrile-acetic acid solution as mobile phase were employed to determine the content of syringin and rutin in 11 species of Saussurea by RP-HPLC. The detection wavelength was 260 nm. The results showed that standard curves of syringin and rutin were linear in the range of 0.0525 microgram-1.05 micrograms and 0.0658 microgram-1.32 micrograms, respectively. The correlation coefficients were 0.9988 and 0.9999. The method provides new indices for quality control of Saussurea.
