Determination of Thyroxine Enantiomers in Human Plasma withNormal High Performance Liquid Chromatography-ChiralComplex Exchange Mobile Phase
正相高效液相色谱-手性配位基交换流动相法测定人血清中的甲状腺素对映体

An HPLC method was developed with normal HPLC chiral complex mobile phase for the separation and determination of D and L thyroxine enantiomers (D and L T4) in human plasma The method includes extraction of thyroxine from plasma and separation of thyroxine enantiomers on HPLC silica column with chiral eluent containing L proline, cupric acetate and triethylamine (TEA) The sensitivity of the method was 0 1 mg/L The precisions of inter day and intra day, linearity, extraction recovery, and stability of T4 enantiomers in plasma and in deproteinized plasma were determined for the validation of the method Baseline enantioseparation of the compounds containing D and L T4 was achieved Meantime we determined the concentrations of D T4 and L T4 in plasma of 15 volunteers with euthyroid, hypothyroid and hyperthyroid symptoms. Liquid chromatographic method based on the described procedure was useful for the determination of D and L thyroxine in patient plasma and for pharmacokinetics investigation