Determination of Hesperidin and Naringin in Fructus Aurantii Immaturus and Fructus Aurantii by Reversed-Phase High Performance Liquid Chromatography
反相高效液相法测定枳实、枳壳中橙皮甙和柚皮甙的含量

An HPLC method was established for the determination of hesperidin and naringin in the Fructus Aurantii Immaturus and Fructus Aurantii from different places. Hesperidin and narigin were separated on a Hypersil ODS1 column with acetonitrile-0.5% acetic acid (volume ratio of 22:78) as the mobile phase and detected at 283 nm. The flow rate was 1.0 mL/min. The column temperature was set at 35 degrees C. The calibration curves were linear in the ranges of 0.060 g/L-0.604 g/L and 0.0125 g/L-0.125 g/L for naringin and hesperidin respectively. The average recoveries were 97.1% for naringin and 95.3% for hesperidin. The method is simple, rapid, accurate and reliable.