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热带亚热带植物学报 2009
Tissue Culture and Rapid Propagation of Enkianthus quinqueflorus Lour
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Abstract:
The rapid propagation of Enkianthus quinqueflouus Lour. had been established by using shoot-tip or stem with auxiliary buds as explants. The modified B5 medium as basal medium was optimum for callus induction. The callus induction rate was up to 100% cultured on B5 medium with 1 mg L-1 2,4-D. The adventitious buds were induced on B5 medium supplemented with 1~2 mg L-1 BA +0.1~0.5 mg L-1 NAA. Propagation coefficient was the highest when adventitious buds were subcultured on modified B5+1 mg L-1 BA+0.5 mg L-1 NAA. The rooting could reach 80% when adventitious buds cultured on 1/2MS+2 mg L-1 IBA, and the survival of plantlet could reach 90% after transferred into perlite bed.