Cloning and Sequence Analysis of a Novel Cis-prenyltransferases Gene from Hevea brasiliensis
巴西橡胶树顺式异戊烯基转移酶基因cDNA克隆及其序列特征分析

Cis-prenyltransferases is the key enzyme to rubber biosynthesis.A cDNA fragment which was highly homologous to the gene encoding cis-prenyltransferases was isolated by screening of a latex subtracted cDNA library.The latex subtracted cDNA library was constructed by suppressive subtractive hybridization(SSH),in which the polyA+RNA of latex from Hevea brasiliensis served as tester,and polyA+RNA of leaves as driver.According to its sequences information,a novel full-length cDNA of 1 156 bp termed R363(GenBank ...