Inhibitory Effects of Ganoderma lucidum Extraction Tissue Culture of Common Banana
灵芝浸出液在香蕉组织培养中的抑菌作用

The experiments of the effects of Ganoderma lucidum(Leyss.ex Fr.) Karst.extract(GLE) on bacterial inhibition in tissue culture of common banana was carried out by adding various concentrations of GLE into MS medium containing 2 mg L-1 BA and 0.1 mg L-1 NAA.Explants from absorptive buds of common banana(Musa sapientum L.) were used to observe the inhibition of bacteria(Escherichia coli I,Saccharomyces cerevisiae,Staphylococcus aureus,Streptomyces griseus and Agrobacterium tumefaciens),the shooting and the growth of plantlets.It was indicated that best bacterial inhibition concentrations of GLE in primary culture and subcultures were 5 or 10 g Ganoderma pieces boiled in 1 000 ml distilled water for two hours.However,GLE showed certain inhibitory effect on shooting,but there was no influence on rooting.