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菌物学报 2011
The anastomosis groups of the corn sheath blight pathogen Rhizoctonia spp. in northeastern China
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Abstract:
Two hundred and eighty-six isolates were obtained from corn sheath blight samples in northeastern China (including Heilongjiang, Jilin and Liaoning provinces). Anastomosis group identification and 5.8S rDNA-internal transcribed spacer (ITS) sequence analysis showed that the isolates belonged to multinucleate Rhizoctonia AG1-IA, AG1-IB, AG1-IC, AG4-HG-I, AG4-HG-III, AG-5,WAG-Z and binucleate Rhizoctonia AG-Ba. Of these, AG-1-IA was the major anastomosis group (AG) (38.46% of total isolates), followed by WAG-Z (26.92%) and AG-5 (24.83%). AG4-HG-III was isolated for the first time from maize in China. 5.8S rDNA-ITS sequence analysis showed that the isolates could be distinctly separated based on their AG types. The isolates belonging to the same AG (or sub-AG) showed high sequence identity.