Optimization of the key factors for processing the granule formulation of Metarhizium anisopliae
金龟子绿僵菌颗粒剂加工的关键因子优化研究

The factors of carriers,accelerant additives and water addition were the key factors in granule formulation processing of Metarhizium anisopliae.After study the influence of these factors on conidial viability,we found that ten examined carriers had no significantly negative effects on conidial germination.Of these,on the contrary,the calcium bentonite,kaolin,pulvistalci,floridin and light calcium carbonate could improve the shelf-life of fungal conidia.Two types of accelerant agents,i.e.,trehalose and tyrosine,could significantly improve the conidia germination with varying rates at 15.12%-24.88% and 15.06%-29.67%,respectively.The effects of water addition on granulation ratio and granulation temperature were obviously correlated.By regression analyses,the suitable addition of water was optimized for reaching more than 90% granulation ratio and obtaining corresponding granulation temperature.The suitable additional quantity of water and suitable granulation temperature for calcium bentonite were 19.1%-25.0% and 50.2-54.5℃,for kaolin 35.0%40.0% and 55.1-56.2℃,for pulvistalci 27.2%-40.0% and 50.4-51.1℃,for floridin 18.3%-25.0% and 51.3-53.6℃ and for light calcium carbonate 40.8%-45.0% and 49.7-56.1℃,respectively.The shelf-life of formulated conidia could be effectively prolonged during storage.