A study on enhancing memory activity constituents from Ganoderma tsugae
松杉灵芝增强记忆活性成分的研究

The latent period experiment, the determination of ChAT (choline acetyltransferase), AchE (acetylcholinesterase), and ACh (acetylcholine), and the preparation of brain tissue slice were used to study capability of enhancing memory activity of mice induced by petroleum ether extract, chloroform extract, methanol extract and water extract of Ganoderma tsugae fruit body and 1-hydroxy-3-methylanthracene-9,10-dione obtained from petroleum ether extract. The results showed that the latent period of high dose methanol extract treatment group, high dose water extract treatment group, high dose chloroform extract treatment group, piracetam treatment group and 1-hydroxy-3-methylanthracene-9,10-dione treatment group were significantly different as compared with the group of pathological model. These groups not only could reduce AchE activity, but also could enhance the ChAT and Ach activity, and 1-hydroxy-3-methylanthracene-9,10-dione had the best effect. The number of nerve cells for water extract treatment group and methanol?extract treatment group were significantly increased, approach the youth?group. The nerve cells were sphere and had obvious?leveling effect. The nerve cells of aged group and model control group were coniform. comparison with the water extract treatment group and methanol?extract treatment group, the number of nerve cells of aged group were significantly decrease. Enhancing memory activity experiment results showed that 1-hydroxy-3-methylanthracene-9,10-dione treatment group and water extract treatment group had excellent enhancing memory activity effect.