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菌物学报 2012
Fast molecular detection of the pathogen of sunflower black stem
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Abstract:
The diseased stems, leaves and faceplates of sunflower with black stem symptom were sampled in Xinjiang Autonomous Region, China. Twenty fungal isolates were finally isolated from the materials. Isolates XJ011 and XJ111 were found to be pathogenic to sunflower plants. ITS sequence analysis indicated the isolates XJ011 and XJ111 were Phoma macdonaldii. A pair of specific primers 320FOR/320REV were designed for rDNA-ITS of P. macdonaldii according to polymorphism region, and it can be used to detect P. macdonaldii specifically with highly sensitivity of 1fg concentration.
