Effects of additives and different culture conditions on cordycepin production by the medicinal fungus Cordyceps militaris
不同培养条件和前体对蛹虫草液体发酵产虫草素的影响

The medicinal fungus Cordyceps militaris produces many active metabolites including cordycepin(3’-deoxyadenosine).The effects of additives and different liquid culture conditions on cordycepin production by C.militaris were investigated.The strain of C.militaris 08Y1 was used for optimizing liquid culture conditions(light and shaking,light and static,dark and shaking,dark and static),and the additives(adenine 1g/L+glycine 16g/L) were added to medium for 16 days.The maximum cordycepin production of 1,015.0mg/L was achieved by culture at dark and shaking with the supplement of additives,and the maximum utilization ratio of adenine reached 98.5%.The results showed that the condition of dark and shaking with the supplement of additives was an effective way for increasing the production of cordycepin.