MOLECULAR DETECTION OF COMPETITIVE COLONIZATION OF AM FUNGI WITHIN FRESH ROOTS
以新鲜根段进行AM真菌的分子检测及竞争性侵染研究

Two species of arbuscular mycorrhizal fungi, Glomus intraradices and Glomus mosseae , were detected in fresh plant roots by means of molecular approach-nested PCR. Roughly extracted DNA from tomato fresh root was amplified by using universal eukaryotic primers LR1-NDL22. The PCR products were diluted and used as template for the second PCR amplification using species specific primers 8.22 and 5.25 combined with LR1 or NDL22 respectively. Two Glomus species were identified specifically by this technique. In a glasshouse experiment, tomato seedlings were mixed-inoculated with G. intraradices and G. mosseae. Four weeks after inoculation, both inoculated fungi were detected in the same infected root fragments. The present study opens the possibility for molecular monitoring the persistence and dissemination of introduced efficient species of AM fungi in natural ecosystem and evaluation of their potential in field application.