Distribution of a specific SCAR marker among Lentinula edodes protoplast monokaryons for strain 135
香菇135菌株特异SCAR标记在其原生质体单核中的分布

Refrigerated mycelium and fresh fruitbody tissue-isolated mycelium of Lentinula edodes strain 135 were used to prepare protoplast monokaryons. Fifty-eight and 83 monokaryons were isolated respectively and their mating types were determined. PCR amplification employing specific primers was used to establish the distribution among the protoplast monokaryons of a SCAR marker specific for strain 135. Protoplast monokaryons segregated into either A1B1 or A2B2 mating type, and the SCAR marker was detected only in the latter. The outcome is a regular distribution pattern that demonstrates the stable inheritance of the SCAR marker and is fundamental to identification of offspring arising from crosses between protoplast monokaryons of this particular mating type and other karyons.