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菌物学报 2009
Sequence and phylogenetic analysis of OPA2-1 region from Alternaria small-spored species
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Abstract:
The OPA2-1 region of small-spored Alternaria species was amplified with specific primer pairs,OPA2-1L/OPA2-1R,all strains produced one single same size band.The analysis of their nucleotide acid sequences indicates that certain differences were found in nucleotide sequences among these species.The phylogenetic tree constructed based on neighbor-joining method shows that these isolates were divided into eight clades.Alternaria gaisen,A.longipes and A.arborescens were clustered into different clades,while iso...