Development of microsatellite markers for Lentinula edodes, using database search and ISSR-suppression PCR methods
数据库搜索及ISSR-抑制PCR法开发香菇微卫星标记

Two methods,database search and ISSR-suppression PCR,were used to isolate microsatellite markers from Lentinula edodes.Twenty-one primer pairs were designed by database search,and eleven of them showed polymorphism.The number of alleles produced by these eleven markers was 3.3 per locus in average.Using method of ISSR-suppression PCR,eight primer pairs were developed and five of them had polymorphism.There were 3 alleles per locus in average produced by these five markers.The results showed that two methods...