Pure culture isolation, cultivation and molecular identification of Armillaria luteo-virens from Tibet Plateau
青藏高原黄绿蜜环菌纯培养菌种的分离培养及分子鉴定

Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer (ITS) region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences. The results demonstrated that the strain isolated from fruitbody tissue of Armillaria luteo-virens was the true pure culture of the fungus. Phylogenetic analysis based on 5.8S/ITS sequence indicated Armillaria luteo-virens has a distant phylogenetic relationship with other genera of Tricholomataceae. Phylogenetic analysis based on two IGS-1 sequences indicated there were significant differences between the IGS-1 sequence of Armillaria luteo-virens and that of other species of Armillaria. Armillaria luteo-virens has a relatively close phylogenetic relationship with some species of Lepiota.