Effects of nitric oxide on production of antioxidant phenolic compounds in Phaeoporus obliquus
一氧化氮对桦褐孔菌抗氧化酚类化合物积累的影响

This study was designed to evaluate the effects of nitric oxide (NO) on the production of phenolic compounds by Phaeoporus obliquus in submerged cultures and their subsequent antioxidant activities. NO was supplemented to the cultures in the form of sodium nitroprusside (SNP) at final concentrations of 0.01mmol/L, 0.1mmol/L and 1mmol/L, respectively, and total phenolic compounds and their subsequent antioxidant activities were determined. Antioxidant activity was expressed as potentials for scavenging DPPH and hydroxyl radicals. The exposure of P. obliquus to SNP at a final concentration of 0.1mmol/L resulted in a highest production of total intracellular and exocellular phenolic compounds, with levels reaching 67.00mg/g and 677.00mg/L, respectively. Both intra- and extracellular phenolic compounds produced at different culture ages all possessed antioxidant activities with higher levels seen in the cultures supplemented with SNP at a concentration of 0.1mmol/L. Thus NO can possibly be used for up-regulating accumulation of phenolic compounds in submerged cultures of P. obliquus.