Study on differential development gene of Pholiota adiposa bv DDRT-PCR
运用mRNA差异显示技术研究黄伞发育相关基因

RNA of different development periods of pholiota adiposa including mycelium,anlage,bud and fruit body(stem and pileus of mushroom)was extracted and treated by RT-PCR.Four fragments(T_(11)G B0304,T_(11)G B0304-2,T_(11)G B0322 and T_(11)C B0310)were isolated,reamplified,purified,cloned and sequenced.After blasting in GenBank,T_(11)G B0322 cDNA fragment was identified to be similar to oxysterol-binding protein.The result of semiquantitive PCR showed that the expression of the T_(11)G B0322 cDNA fragment was different in different periods.This showed that gene fragment might be associated with differentiation of Ph.adiposa.