Functional analysis of gpd-Le and ras-Le promoters from Lentinus edodes
香菇印gpd-Le和ras-Le启动子的功能分析

The expression vectors pLg-gfp and pLr-gfp containing gpd-Le and ras-Le promoter fragments respectively from Lentinus edodes were constructed for detecting its functional activity by the fusion of promoter fragments to the reporter gfp gene.Co-transformation of plasmid pLe-gfp and pLr-gfp with plasmid pCc1001 respectively,which harbored the complementary gene trpl were conducted by the PEG-mediated protoplast transformation of the oidia of LT2,a tryptophan auxotrophic strain of Coprinus cinereus,The putative trp+ transformants were obtained from the selective regeneration medium and confirmed by PCR detection,Southern blotting and green fluorescence analysis. The results indicated that gfp gene expression in Coprinus cinereus. Strong green fluoescence acitivity was observed in the mycilia of pLg-gfp transformants of Coprinus cinereus LT2 under the fluorescent electronic microscope.However,the ras-Le promoter could not drive the gfp gene expression in Coprinus cinereus. No green fluorescence acitivity was observed in the mycelia of pLr-gfp transformants of Coprinus cinereus LT2.