The analysis of crude triterpenoids and flavonoids contents, antioxidative and antitumor activities of the crude extracts from culture broth of Volvariella volvacea
草菇培养物中粗三萜和黄酮含量及抗氧化抗肿瘤活性研究

The spawns of Volvariella volvacea were inoculated into potato dextrose liquid media of triangle-flasks and cultured by constantly shaking for 7 days. Mycelia and cultured filtrates were extracted by petroleum ether (PE),ethyl acetate (EA),ethanol (Eth),respectively,and the different organic portions were used to prepare the extracts of secondary metabolites. The component analysis indicated that there were crude triterpenoids and flavonoids in all the extracts,but the contents of crude triterpenoids and flavonoids in mycelia extracts were higher than those in cultured filtrates. There was the most crude triterpenoids content (17%) in the mycelia extracts obtained by PE. However,the most flavonoids content (9.31%) was in the mycelia extracts obtained by EA. Antioxidative assay showed three kinds of secondary metabolites possessed antioxidative activities,but the activities of the extracts from EA were visibly higher than those from PE. The mycelia extracts from EA and Eth had the highest antioxidative activities. MTT assay suggested that the extracts could inhibit the proliferation of BGC cancer cells and exhibit higher antitumor activitives,and there was a dose-effect relationship between the extracts concentration and the antitumor activitives.