Cloning of a gene encoding lipase from Thermomyces lanuginosus and effective expression of the gene in Pichia pastoris
疏棉状嗜热丝孢菌脂肪酶基因的克隆及其在毕赤酵母中的高效表达

Thermomyces lanuginosus produces an extracellular lipase that is important for industrial applications. Based on published DNA sequence, we cloned the full length genomic DNA and cDNA of the lipase using PCR and RT-PCR methods. DNA sequencing revealed that the genomic DNA of the T. lanuginosus lipase gene has an open reading frame of 876bp encoding a protein lipase precursor of 292 amino acid residues, and three introns. And the cDNA of lipase gene we cloned is identical to the registered lipase gene of The...