Application of PCR-DGGE to analysis of microbial community structure in aerobic unit of A2/O process
PCR-DGGE技术解析A2/O工艺好氧单元中微生物群落结构

The dynamic process and the microbial community structure in the aerobic activated sludge system of the industrial wastewater treatment were traced by the PCR-DGGE technology.The results show that the microbial community structure changes with the water quality in the system and becomes stable as the incubation time increasing.The microbial community structure is mainly composed of five categories which respectively have close relationships with the γ,δ,α,ε Proteobacterias,Bacilli.γ Proteobacterium is the main microbial community,which including Pseudomonas sp.,Rheinheimera sp.,Citrobacter sp.,Klebsiella sp.,Enterbacteriaceae,Stenotrophomonas maltophilia,Acinetobacter.Five bacteria including uncultured Pseudomonas sp.,Halobacillus sp.,Pseudomonas sp.,Pseudomonas stutzeri,Acinetobacter sp.can exist stably in the system and become dominant microorganisms.So the effect of wastewater treatment can be improved by improving quantity and quality of the dominant microorganisms in the system.