Effects of nutrients on ferrous ion biooxidation
营养成分对Fe2+生物氧化的影响

A strain of Acidithiobacillus ferrooxidans(A.f.a) which was from acid mine drainage of Yunfu mine in Guangdong,was obtained by enrichment cultivation using 9K medium,on a rotary shaker at 160 r/min and a constant temperature of 29℃.The effects of nutrients such as NH+4-N,PO3-4-P,Mg2+,Ca2+ and Cu2+ on ferrous biooxidation by the strain A.f.a were studied with batch experiments.The results show that the ferrous biooxidation rate is tended to increase first and then decrease with increasing NH+4-N,PO3-4-P,Ca2+ or Cu2+ concentration.Their optimum concentrations are 84.8,35.6,2.4 and 254.5 mg/L,respectively.With increasing Mg2+ concentration,the ferrous biooxidation rate increases nearly to stability,and the limiting concentration is 88.8 mg/L.The results can be used to improve ferrous biooxidation by controlling the conditions of nutrients.