New technique of seedling cultivation of Pinctada martensii(Dunker)
马氏珠母贝种苗规模化繁育新技术及工艺

Key steps pertaining to artificial larviculture of Pinctada martensii (Dunker) were studied. Results showed that: (1) compared with the groups with water replacement, the survival rates and shell length daily growth of D-shape larvae and umboned larvae, and the survival rate and shell length daily growth of juvenile for the groups without water replacement were increased by 15.3%, 259.6%, 33.3%, 34.2%, 186.5%, and 12.4%, respectively (P<0.01); (2) the daily growth of juvenile shell length of the group with two-time attachment board placing was greater than that of the group with one-time attachment placing, which the daily growth of juvenile shell length was smaller and was significantly different than those for above groups (P<0.01). The evenness of the same batch of juveniles of the multiple-time attachment board placing group was higher than that for the one-time attachment board placing group, and juvenile cultivation rate, juvenile survival rate and larval collection rate were 32.5%, 19.3% and 35% higher than those of the one-time attachment board placing group, respectively; (3) the juvenile survival rate, cultivation rate, and shell length daily growth of the group fed with shrimp pond water were 28.1%, 47.2% and 35.9% higher than those of the group fed with 50% autolyzed yeast plus 50% chlorella, respectively, whereas the survivals for the dark-dried juveniles that were fed with these two diets were not significantly different. It was concluded that these techniques, such as closed larviculture without water replacement, multiple attachment board placing, and feeding with living diets in shrimp pond water, had great impact on efficiently cultivating healthy seedlings of P. martensii.