Preparation and in vitro releasing characteristics of recombinant antimicrobial peptides sodium alginate microcapsules
重组抗菌肽海藻酸钠微囊制备与体外释放特征研究

In this paper, the recombinant antimicrobial peptides (penaeidin 3-2) were purified with affinity chromatography, and recombinant antimicrobial peptides sodium alginate microcapsules were prepared by using coacervation technology. The morphology and encapsulation rate were introduced as indexes of microcapsules formulation to optimize the preparation technology. The in vitro releasing characteristics of the optimal microcapsules was investigated and the results showed that these ball-shaped microcapsules with a diameter of 1.1mm and an encapsulation rate of 83.87% were prepared with 1.5% calcium chloride and 2.0% sodium alginate. The releasing rate of recombinant antimicrobial peptides after standing for 2 h in simulated gastric fluid (pH 2.0) was less than 14%. In contrast, after standing in simulated intestinal fluid (pH 7.8) for 5 h, the releasing rate of recombinant antimicrobial peptides reached 98%. These data suggested that the intestinal-lysis microcapsules have strong resistance to gastric fluid and can be used as a sustained-release and controlled-release reagent, providing the experimental basis for oral drug delivery system in aquaculture.